Background: Transfusion of blood and blood components, as a specialized modality of patient management, saves millions of lives worldwide each year and reduce morbidity. It is well-known that blood transfusion is associated with a large number of complications. Objective: The objective of the present study was to assess the status of transfusion transmitted infections (TTI) among the apparently healthy donors so as to increase the awareness of complications of blood transfusion and make the clinicians more vigilant with regard to judicious use of blood. Methods: A total of 21,966 units of donor’s blood were screened from January 2007 to December 2010 at blood bank of Sir Salimullah Medical College, Mitford Hospital, Dhaka. All the samples were screened for hepatitis B surface antigen (HBsAg), hepatitis C virus (HCV), human immunodeficiency virus (HIV) 1 and 2, venereal disease research laboratory test (VDRL) and malaria. All healthy blood donors were 18 to 60 years old. Result: Prevalence of hepatitis B virus (HBV), HCV, HIV and syphilis were 2.19, 0.25, 0.06 and 0.15% respectively. No blood donor tested showed positivity for malarial parasite. With the implementation of strict donor selection criteria and use of sensitive screening test, it may be possible to reduce the incidence of TTI in the Bangladeshi population.