Citation Information :
Jahan M, Tabassum S, Nessa A, Shahera U, Munshi S, Alam S. IP-10, p53, and Foxp3 Expression in Hepatocytes of Chronic Hepatitis B Patients with Cirrhosis and Hepatocellular Carcinoma. Euroasian J Hepatogastroenterol 2016; 6 (2):149-153.
Aim: Elucidating differences in gene expression may be useful in understanding the molecular pathogenesis
and for developing specific markers for the outcome of hepatitis B virus (HBV) infection. In the
present study, expressions of host gene interferon gamma-inducible protein (IP-10), p53, and Foxp3
were studied in hepatocytes of patients with chronic HBV infection to determine a possible link between
selected host gene expression and the outcome of HBV infection.
Materials and methods: The study was conducted in 60 patients with chronic HBV infection and
they were divided into four groups: HBV-positive cirrhosis (n = 15), HBV-negative cirrhosis (n = 15),
HBV-positive hepatocellular carcinoma (HCC) (n = 15) and HBV-negative HCC (n = 15). Total messenger
ribonucleic acid (mRNA) extraction was done followed by complementary deoxyribonucleic
acid (cDNA) synthesis, and finally gene expression was performed using real-time polymerase chain
reaction (PCR) technique.
Results: I P-10 a nd p 53 g ene e xpressions w ere l ower i n H BV-positive c irrhosis, a nd F oxp3 g ene
expression was upregulated in HBV-positive cirrhosis in comparison to HBV-negative cirrhosis. The
expressions of all the three genes were upregulated among HBV-positive HCC in comparison to HBVnegative
HCC. The expression of IP-10, p53, and Foxp3 genes was upregulated in HBV-positive HCC
in comparison to HBV-positive cirrhosis.
Conclusion: This study indicates that there are variations in the expression of the selected genes
among cirrhosis and HCC patients with or without HBV. All the three selected genes were more or
less upregulated in HBV-positive HCC patients, but only Foxp3 expression was upregulated in HBVpositive
cirrhosis. These three particular genes may have a role in the molecular pathogenesis and
clinical outcome of HBV-positive cirrhosis and HCC patients. These aspects need further evaluation
by studies with larger numbers of cirrhosis and HCC patients.
Aandahl EM, Michaelsson J, Moretto WJ, Hecht FM, Nixon DF. Human CD4+ CD25+ regulatory T cells control T-cell responses to human immune deficiency virus and cytomegalovirus antigens. J Virol 2004 Mar;78(5):2454-2459
Hepatitis B and hepatocellular carcinoma. Hepatology 2009 May;49(Suppl 5):56-60
HBV life cycle and novel drug targets. Hepatol Int 2011 Jun;5(2):644-653
Hepatitis B virus infection: understanding its epidemiology, course, and diagnosis. Cleve Clin J Med 2008 Dec;75(12):881-889
Genetic susceptibility in chronic viral hepatitis. Antivir Res 2001 Nov;52(2):113-116
A population-based study to investigate host genetic factors associated with hepatitis B infection and pathogenesis in the Chinese population. BMC Infect Dis 2008 Jan;8(1):12-15
Expression of CXC chemokine IP10 in patients with chronic hepatitis B. HPDI 2008;7:45-50
Activated IL-23/IL-17 pathway closely correlates with increased Foxp3 expression in livers of chronic hepatitis B patients. BMC Immunol 2011;12:25
The role of chemokines as inflammatory mediators in chronic hepatitis C virus infection. J Viral Hepatol 2007 Oct;14(10):675-687
Live or let die: the cell’s response to p53. Nat Rev Cancer 2002 Aug;2(8):594-604
Importance of p53 for cancer onset and therapy. Anticancer Drugs 2006 Aug;17(7):725-732
Circulating and liver resident CD4+ CD25+ regulatory T cells activity influence the antiviral immune response and disease progression in patients with hepatitis B. J Immunol 2006 Jul;177(1):739-747
Differential cellular gene expression induced by hepatitis B and C viruses. Biochem Biophys Res Commun 2003 Jan;300(2):443-447
Differential gene expression between chronic hepatitis B and C hepatic lesion. J Gastroenterol 2001 Mar;120(4):955-966
Characterization of liver-cirrhosis nodules by analysis of geneexpression profiles and patterns of allelic loss. J Hum Genet 2004;49(5):246-255. IP-10, p53, and Foxp3 Expression in Hepatocytes of Chronic Hepatitis B Patients with Cirrhosis and Hepatocellular Carcinoma Euroasian Journal of Hepato-Gastroenterology, July-December 2016;6(2):149-153 153 EJOHG 16. Anders RA. cDNA microarray analysis of macro regenerative and dysplastic nodules in end-stage hepatitis C virus-induced cirrhosis. Am J Pathol 2003 Mar;162(3):991-1000
Genome-wide analysis of gene expression in human hepatocellular carcinomas using cDNA microarray: identification of genes involved in viral carcinogenesis and tumor progression. Cancer Res 2001 Mar;61(5):2129-2137
Epidemiology, risk factors, and natural history of hepatocellular carcinoma. Ann N Y Acad Sci 2002 Jun;963: 13-20
Expansion of peripheral and intratumoral regulatory T-cells in hepatocellular carcinoma: a case-control study. Indian J Pathol Microbiol 2011 Jul-Sep;54(3):448-453.