Hepatitis B Core Antigen in Hepatocytes of Chronic
Hepatitis B: Comparison between Indirect
Immunofluorescence and Immunoperoxidase Method
Munira Jahan, Shahina Tabassum, Mamun Al-Mahtab, Afzalun Nessa, Chowdhury Mohammad Shamim Kabir, Mohammad Kamal, Julio Cesar Aguilar
Citation Information :
Jahan M, Tabassum S, Al-Mahtab M, Nessa A, Mohammad Shamim Kabir C, Kamal M, Cesar Aguilar J. Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method. Euroasian J Hepatogastroenterol 2015; 5 (1):7-10.
Background: Hepatitis B virus (HBV) infection has many faces. Precore and core promoter
mutants resemble inactive carrier status. The identification of hepatitis B core antigen (HBcAg) in
hepatocytes may have variable clinical significance. The present study was undertaken to detect
HBcAg in chronic hepatitis B (CHB) patients and to assess the efficacy of detection system by indirect
immunofluorescence (IIF) and indirect immunoperoxidase (IIP).
Materials and methods: The study was done in 70 chronic HBV-infected patients. Out of 70
patients, eight (11.4%) were hepatitis B e antigen (HBeAg) positive and 62 (88.57%) were HBeAg
negative. Hepatitis B core antigen was detected by indirect immunofluorescence (IIF) and indirect
immunoperoxidase (IIP) methods in liver tissue.
Results: All HBeAg positive patients expressed HBcAg by both IIF and IIP methods. Out of 62
patients with HBeAg-negative CHB, HBcAg was detected by IIF in 55 (88.7%) patients and by IIP
in 51 (82.26%) patients. A positive relation among viral load and HBcAg detection was also found.
This was more evident in the case of HBeAg negative patients and showed a positive relation with
HBV DNA levels.
Conclusion: Hepatitis B core antigen can be detected using the IIF from formalin fixed paraffin block
preparation and also by IIP method. This seems to reflect the magnitudes of HBV replication in CHB.
Mehedi SR, Pophali A, Al- Abdul Rahim KA. Prevalence of hepatitis B and C and blood donors. Saudi Med J 2000;21: 942-944
Introduction to chronic hepatitis B infection. Am J Gastroenterol 2006;101(s1):S1-S6
Epidemiology and prevention of hepatitis B virus infection. Int J Med Sci 2005;2(1):50-57
Chronic hepatitis B virus infection in Asian countries. J Gastroenterol Hepatol 2000;15(12):1356-1361
Clinical use of liver biopsy for the diagnosis and management of inactive and asymptomatic hepatitis B virus carriers in Bangladesh. J Med Virol 2010;82:1350-1354
Clinical profile: Prognostic index in hepatocellular carcinoma. Bangladesh Med Res Bull 1991;XVII:49-62
Hepatitis B virus is the leading cause of cirrhosis of liver in Bangladesh. Hepatol Int 2007;1(1):120
Alam K, Khan M. Characteristics of treatment naïve chronic hepatitis B in Bangladesh: Younger populations are more affected: HBeAg-negatives are more advanced. Saudi J Gastroenterol 2008;14(1):15-19
Hepatology: A clinical text book 2009;5:59-61
World-wide epidemiology of HBeAg-negative chronic hepatitis B and associated precore and core promoter variants. J Viral Hepat 2002 Jan;9(1):52-61
Corrections to AASLD guidelines on chronic hepatitis B. Hepatol 2007;45(6):506-539
Hepatitis B core antigen expression pattern reflects the response to antiviral treatment. J Gastroenterol Hepatol 2006;21:977-981
The Degree of Hepatocyte Cytoplasmic Expression of Hepatitis B Core Antigen correlates with Histologic Activity of Liver Disease in the Young Patients with Chronic Hepatitis B Infection. J Korean Med Sci 2006 Apr;21(2):279-283
Diagnosis of hepatitis B virus infection through serological and virological markers. Expert Rev Gastroenterol Hepatol 2008;2(4):553-562
Natural History of Hepatitis B Virus Infection: An Update for Clinicians. Mayo Clin Proc 2007;82(8):967-975
The clinical presentation of chronic hepatitis B virus infection in Asian Americans: a single center retrospective study. J Clin Gastroenterol 2010;44(5):364-370
Membrane staining for hepatitis B surface antigen on hepatocytes: a sensitive and specific marker of active viral replication in hepatitis B. J Clin Pathol 1995;48(5): 470-473
High HBcAg Expression in Hepatocytes of Chronic Hepatitis B Patents in Bangladesh. Euroasian Journal of Hepato-Gastroenterology 2012;2(2):63-69
Immunohistological study of intrahepatic expression of hepatitis B core and E antigens in chronic type B hepatitis. J Clin Pathol 1992;45(9):791-795
Clinical Use of Liver Biopsy for the Diagnosis and Management of Inactive and asymptomatic Hepatitis B Virus Carriers in Bangladesh. J Med Virol 2010;82(8):1350-1354
Treatment of hepatitis B virus-infected patients: utility of therapeutic recommendations in developing countries. Expert Opin Pharmacother 2009;10(10):1605-1614
The majority of hepatitis B carriers are not on regular surveillance in Singapore. Singapore Med J 2004;45(9):423-426
Current treatment indications and strategies in chronic hepatitis B virus infection. World J Gastroenterol 2008;14(45): 6902-6910
Is there a meaningful serum hepatitis B virus DNA cutoff level for therapeutic decisions in hepatitis B e antigen-negative chronic hepatitis B virus infection? Hepatol 2008 Nov;48(5): 1451-1459.