Euroasian journal of hepato-gastroenterology

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VOLUME 5 , ISSUE 1 ( January-June, 2015 ) > List of Articles

ORIGINAL ARTICLE

Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method

Munira Jahan, Shahina Tabassum, Mamun Al-Mahtab, Afzalun Nessa, Chowdhury Mohammad Shamim Kabir, Mohammad Kamal, Julio Cesar Aguilar

Citation Information : Jahan M, Tabassum S, Al-Mahtab M, Nessa A, Mohammad Shamim Kabir C, Kamal M, Cesar Aguilar J. Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method. Euroasian J Hepatogastroenterol 2015; 5 (1):7-10.

DOI: 10.5005/jp-journals-10018-1120

License: CC BY-NC 4.0

Published Online: 01-06-2017

Copyright Statement:  Copyright © 2015; The Author(s).


Abstract

Background: Hepatitis B virus (HBV) infection has many faces. Precore and core promoter mutants resemble inactive carrier status. The identification of hepatitis B core antigen (HBcAg) in hepatocytes may have variable clinical significance. The present study was undertaken to detect HBcAg in chronic hepatitis B (CHB) patients and to assess the efficacy of detection system by indirect immunofluorescence (IIF) and indirect immunoperoxidase (IIP). Materials and methods: The study was done in 70 chronic HBV-infected patients. Out of 70 patients, eight (11.4%) were hepatitis B e antigen (HBeAg) positive and 62 (88.57%) were HBeAg negative. Hepatitis B core antigen was detected by indirect immunofluorescence (IIF) and indirect immunoperoxidase (IIP) methods in liver tissue. Results: All HBeAg positive patients expressed HBcAg by both IIF and IIP methods. Out of 62 patients with HBeAg-negative CHB, HBcAg was detected by IIF in 55 (88.7%) patients and by IIP in 51 (82.26%) patients. A positive relation among viral load and HBcAg detection was also found. This was more evident in the case of HBeAg negative patients and showed a positive relation with HBV DNA levels. Conclusion: Hepatitis B core antigen can be detected using the IIF from formalin fixed paraffin block preparation and also by IIP method. This seems to reflect the magnitudes of HBV replication in CHB.

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  1. Mehedi SR, Pophali A, Al- Abdul Rahim KA. Prevalence of hepatitis B and C and blood donors. Saudi Med J 2000;21: 942-944
  2. Introduction to chronic hepatitis B infection. Am J Gastroenterol 2006;101(s1):S1-S6
  3. Epidemiology and prevention of hepatitis B virus infection. Int J Med Sci 2005;2(1):50-57
  4. Chronic hepatitis B virus infection in Asian countries. J Gastroenterol Hepatol 2000;15(12):1356-1361
  5. Clinical use of liver biopsy for the diagnosis and management of inactive and asymptomatic hepatitis B virus carriers in Bangladesh. J Med Virol 2010;82:1350-1354
  6. Clinical profile: Prognostic index in hepatocellular carcinoma. Bangladesh Med Res Bull 1991;XVII:49-62
  7. Hepatitis B virus is the leading cause of cirrhosis of liver in Bangladesh. Hepatol Int 2007;1(1):120
  8. Alam K, Khan M. Characteristics of treatment naïve chronic hepatitis B in Bangladesh: Younger populations are more affected: HBeAg-negatives are more advanced. Saudi J Gastroenterol 2008;14(1):15-19
  9. Hepatology: A clinical text book 2009;5:59-61
  10. World-wide epidemiology of HBeAg-negative chronic hepatitis B and associated precore and core promoter variants. J Viral Hepat 2002 Jan;9(1):52-61
  11. Corrections to AASLD guidelines on chronic hepatitis B. Hepatol 2007;45(6):506-539
  12. Hepatitis B core antigen expression pattern reflects the response to antiviral treatment. J Gastroenterol Hepatol 2006;21:977-981
  13. The Degree of Hepatocyte Cytoplasmic Expression of Hepatitis B Core Antigen correlates with Histologic Activity of Liver Disease in the Young Patients with Chronic Hepatitis B Infection. J Korean Med Sci 2006 Apr;21(2):279-283
  14. Diagnosis of hepatitis B virus infection through serological and virological markers. Expert Rev Gastroenterol Hepatol 2008;2(4):553-562
  15. Natural History of Hepatitis B Virus Infection: An Update for Clinicians. Mayo Clin Proc 2007;82(8):967-975
  16. The clinical presentation of chronic hepatitis B virus infection in Asian Americans: a single center retrospective study. J Clin Gastroenterol 2010;44(5):364-370
  17. Membrane staining for hepatitis B surface antigen on hepatocytes: a sensitive and specific marker of active viral replication in hepatitis B. J Clin Pathol 1995;48(5): 470-473
  18. High HBcAg Expression in Hepatocytes of Chronic Hepatitis B Patents in Bangladesh. Euroasian Journal of Hepato-Gastroenterology 2012;2(2):63-69
  19. Immunohistological study of intrahepatic expression of hepatitis B core and E antigens in chronic type B hepatitis. J Clin Pathol 1992;45(9):791-795
  20. Clinical Use of Liver Biopsy for the Diagnosis and Management of Inactive and asymptomatic Hepatitis B Virus Carriers in Bangladesh. J Med Virol 2010;82(8):1350-1354
  21. Treatment of hepatitis B virus-infected patients: utility of therapeutic recommendations in developing countries. Expert Opin Pharmacother 2009;10(10):1605-1614
  22. The majority of hepatitis B carriers are not on regular surveillance in Singapore. Singapore Med J 2004;45(9):423-426
  23. Current treatment indications and strategies in chronic hepatitis B virus infection. World J Gastroenterol 2008;14(45): 6902-6910
  24. Is there a meaningful serum hepatitis B virus DNA cutoff level for therapeutic decisions in hepatitis B e antigen-negative chronic hepatitis B virus infection? Hepatol 2008 Nov;48(5): 1451-1459.
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