Citation Information :
Tabassum S, Sultana N, Ullah Munshi S, Hossain M, Imam A. Nucleoside Analog-treated Chronic Hepatitis B Patients showed Reduced Expression of PECAM-1 Gene in Peripheral Blood Mononuclear Cells in Bangladesh. Euroasian J Hepatogastroenterol 2014; 4 (2):87-91.
Background and aim: Assessment of therapeutic response is important for monitoring the prognosis
and to take decision for cessation of nucleoside analogues therapy in chronic hepatitis B patients. In
addition to serum alanine aminotransferase (ALT), hepatitis B virus (HBV) deoxyribonucleic acid (DNA)
load and HBeAg status, identification of molecular markers associated with host immune response
would be essential to assess therapeutic response. In this regard the current study was performed
with the aim to detect expression of platelet endothelial cell adhesion molecule (PECAM)-1 gene in
peripheral blood monocytes (PBMCs) of treated chronic hepatitis B patients and also to correlate
expression of this gene with serum HBV DNA load and serum ALT levels.
Materials and methods: The study analyzed 60 chronic hepatitis B (CHB) patients, including 30
untreated and 30 nucleoside analogs treated and 10 healthy controls. PECAM-1 gene expression/
transcripts were detected by conventional RT-PCR.
Results: The expression PECAM-1 mRNA in the PBMCs of CHB patients was significantly higher in
untreated (3.17 ± 0.75) than the treated patients (1.64 ± 0.29) (p < 0.01). Expression of PECAM-1 was
positively correlated with serum ALT levels of both untreated (r = 0.580) and treated (r = 0.566) CHB
patients. Moreover, in both untreated and treated groups, these gene expressions were positively
correlated to serum HBV DNA load with the correlation coefficient r = 0.545 and r = 0.591 respectively.
Conclusion: PECAM-1 may be used as a biomarker for assessment of inflammatory activity as well
as therapeutic response in CHB patients.
Signal transduction pathways mediated by PECAM-1. New roles for an old molecule in platelet and vascular biology. Arterioscler Thromb Vasc Biol 2003 Jun 1;23(6):953-964
Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1-deficient mice. J Clin Invest 2002 Feb;109(3):383-392
Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest 1968;21 (Suppl):77-89
Impaired endothelial nitric oxide synthase activity associated with enhanced caveolin binding in experimental cirrhosis in the rat Gastroenterology 1999 Nov;117(5):1222-1228
A treatment algorithm for the management of chronic hepatitis B virus infection in the United States: 2008 update. Clin Gastroenterol Hepatol 2008 Dec;6(12):1315-1341
Mini-review Transendothelial migration of leukocytes: through the front door or around the side of the house? Eur J Immunol 2004 Nov;34(11):2955-2963.