Euroasian journal of hepato-gastroenterology

Register      Login

VOLUME 3 , ISSUE 1 ( January-June, 2013 ) > List of Articles


Hepatocyte Transplantation

Shimaa Attia Atta

Citation Information : Attia Atta S. Hepatocyte Transplantation. Euroasian J Hepatogastroenterol 2013; 3 (1):59-63.

DOI: 10.5005/jp-journals-10018-1065

License: CC BY-NC 4.0

Published Online: 01-08-2017

Copyright Statement:  Copyright © 2013; The Author(s).


Orthotopic liver transplantation is an established treatment for patients with severe acute and end-stage chronic liver disease. The shortage of donor organs continues to be the rate-limiting factor for liver transplantation throughout the world. Hepatocyte transplantation is a promising treatment for several liver diseases and can, also, be used as a ‘bridge’ to liver transplantation in cases of liver failure.

PDF Share
  1. Moon DK, Lee SG. Liver transplantation. Gut and Liver 2009;3:145-65
  2. Clinical applications of hepatocyte transplantation. World J Gastroenterol 2009;15:2074-77
  3. Cell-based therapies for metabolic liver disease. Mol Genet Metab 2008;95:3-10
  4. Hepatocyte transplantation in animal models. Liver Transpl 2009;15:7-14
  5. Review of hepatocyte transplantation. J Hepatobiliary Pancreat Surg 2009;16:97-100
  6. Transplanted human amniotic membrane-derived mesenchymal stem cells ameliorate carbon tetrachloride-induced liver cirrhosis in mouse. Plos One 2011;2: e16789
  7. Hepatocyte transplantation: A review of laboratory techniques and clinical experience. Surgeon 2007;3:155-64
  8. Hepatocyte cryopreservation: Is it time to change the strategy? World J Gastroenterol 2010;16:1-14
  9. Toward the survival and function of xenogeneic hepatocyte grafts. Liver Transpl 2005;11: 39-50
  10. Route of hepatocyte delivery affects hepatocyte engraftment in the spleen. Transplantation 2003;76:732-34
  11. Hepatocyte transplantation in rats with decompensated cirrhosis. Hepatology 2000;31: 851-57. Table 1: Adult hepatocytes transplantation in chronic liver disease and FHF2 Patients Viable cell range Outcome Died/Alive/LT References Chronic liver disease 20 1.7 × 107 Autotransplant 10 6.0 × 108 – 35 Allotransplant 10 4/6/3 • Alcohol 5 Not available 2/3/- 50 • 1 antitrypsin deficiency 1 2.2 × 107 /1/1 40 • HCV-related 1 7.5 × 106 1/-/- 40 • Others 31 5.0 × 108-2.0 × 109 1/2/2 13 Fulminant hepatic failure 22 13/9/7 Viral (HSV and HBV) 6 1.2 × 108-3.0 × 1010 3/3/2 50 40 Drug-related 10 2.8 × 107-3.9 × 1010 8/2/2 40,37 Idiopathic 42 1.8 × 108-4.0 × 109 1/3/3 50 Others 2 1.7 × 108-4.9 × 108 1/1/- 38 LT: Liver transplantation; HCV: Hepatitis C virus; HSV: Herpes simplex virus; HBV: Hepatitis B virus; 1Cryptogenic cirrhosis (n = 1), idiopathic fibrosis (n = 1) and liver transplant recipient (n = 1); 2Mushroom poisoning (n = 1) and trisegmentectomy (n = 1). Hepatocyte Transplantation Euroasian Journal of Hepato-Gastroenterology, January-June 2013;3(1):59-63 63 EJOHG 12. Baccarani U, Adani GL, Sanna A, et al. Portal vein thrombosis after intraportal hepatocytes transplantation in a liver transplant recipient. Transpl Int 2005;18:750-54
  12. Hepatocyte transplantation using biodegradable matrices in ascorbic aciddeficient rats: Comparison with heterotopically transplanted liver grafts. Transplantation 2001;71:1226-31
  13. Suppression of ICE and apoptosis in mammary epithelial ceils by extracellular matrix. Science 1995;267:891-93
  14. Disruption of epithelial cell-matrix interactions induces apoptosis. J Cell Biol 1994;124:619-26
  15. The drawbridge phenomenon: Representational reasoning or perceptual preference? Dev Psychol 1999;35:427-35
  16. Treatment of fulminant liver failure by transplantation of microencapsulated primary or immortalized xenogeneic hepatocytes. Xenotransplantation 2005;12:457-64
  17. Coencapsulation of hepatocytes and bone marrow cells: In vitro and in vivo studies. Biotechnol Annu Rev 2006;12:137-51
  18. Transdifferentiation of bioencapsulated bone marrow cells into hepatocyte-like cells in the 90% hepatectomized rat model. Liver Transpl 2006;12:566-72
  19. Intrasplenic transplantation of encapsulated hepatocytes decreases mortality and improves liver functions in fulminant hepatic failure from 90% partial hepatectomy in rats. Transplant 2005;79:783-90
  20. Preincubation of rat and human hepatocytes with cytoprotectants prior to cryopreservation can improve viability and function upon thawing. Liver Transpl 2006;12:165-77
  21. Hepatocyte transplantation in the treatment of liver diseases future seems bright after all. Pediatr Transplant 2008;12:4-5
  22. Cryopreservation of primary human hepatocytes: The benefit of trehalose as an additional cryoprotective agent. Liver Transpl 2007;13: 38-45
  23. Cryopreservation of isolated human hepatocytes for transplantation: State of the art. Cryobiology 2006;53:149-59
  24. Treatment of liver failure in rats with end-stage cirrhosis by transplantation of immortalized hepatocytes. Hepatol 2002;36:386-94
  25. Construction of a nontumorigenic rat hepatocyte cell line for transplantation: Reversal of hepatocyte immortalization by site-specific excision of the SV40 T antigen. J Hepatol 2000;33:701-08
  26. Development of cell therapy strategies to overcome copper toxicity in the LEC rat model of Wilson disease. Regen Med 2008;3:165-73
  27. Irradiation as preparative regimen for hepatocyte transplantation causes prolonged cell cycle block. Int J Radiat Biol 2008;84:285-98
  28. Selective portal vein embolization and ligation trigger different regenerative responses in the rat liver. Hepatology 2008;47:1615-23
  29. Fulminant hepatic failure in rats: Survival and effect on blood chemistry and liver regeneration. Hepatol 1996;24:1452-59
  30. Portal branch ligation induces efficient retrovirus mediated gene delivery in rat liver. J Gene Med 2004;6:507-13
  31. Humanized mice in translational biomedical research. Nat Rev Immunol 2007;7:118-30
  32. High-yield preparation of isolated rat liver parenchymal cells: A biochemical and fine structural study. J Cell Biol 1969;43:506-20
  33. Laparoscopyassisted creation of a liver failure model in pigs. Cell Transplant 2008;17:187-93
  34. Hepatocyte transplantation in man. Transplant Proc 1992;24:3052-53
  35. Human fetal hepatocyte transplantation in patients with fulminant hepatic failure. Transplantation 1994;58:951-52
  36. Permanent access to the portal system for cellular transplantation using an implantable port device. Liver Transpl 2004;10:1213-15
  37. Human hepatocyte transplantation: Worldwide results. Transplantation 2006;82:441-49
  38. Successful ex vivo gene therapy directed to liver in a patient with familial hypercholesterolaemia. Nat Genet 1994;6:335-41
  39. Transplantation of human hepatocytes. Transplant Proc 1997;29:2103-06
  40. Liver cell transplantation for the treatment of inborn errors of metabolism. J Inher Metab Dis 2008;31:164-72
  41. Sustained engraftment and tissue enzyme activity after liver cell transplantation for argininosuccinate lyase deficiency. Gastroenterol 2006;130: 1317-23
  42. Hepatocyte Transplantation: Clinical experience and potential for future use. Cell Transplant 2006;15:105-10
  43. Hepatocyte transplantation for liver-based metabolic disorders. J Inherit Metab Dis 2006;29:431-35
  44. Kupffer cells participate in early clearance of syngeneic hepatocytes transplanted in the rat liver. Gastroenterol 2002;123:1677-85
  45. Monocrotaline promotes transplanted cell engraftment and advances liver repopulation in rats via liver conditioning. Hepatol 2006;44: 1411-20
  46. Hepatic sinusoidal vasodilators improve transplanted cell engraftment and ameliorate microcirculatory perturbations in the liver. Hepatol 2002;35:1320-28
  47. Liver cell transplantation: The road to clinical application. J Lab Clin Med 2001;138:298-312
  48. Principles of therapeutic liver repopulation. J Inherit Metab Dis 2006;29:421-25
  49. Hepatocyte transplantation for the treatment of human disease. Semin Liver Dis 1999;19:39-48.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.