Euroasian Journal of Hepato-Gastroenterology

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VOLUME 2 , ISSUE 2 ( July-December, 2012 ) > List of Articles

ORIGINAL ARTICLE

Structural Changes and Gross Pathology of Gut and associated Organs of Mice on Chronic Exposure to Pan Masala Plain and its Blend (Pan Masala Tobacco): A Review and an Experimental Study

Suresh Kumar Nigam, Huthi Venkatakrishna Bhatt

Citation Information : Kumar Nigam S, Venkatakrishna Bhatt H. Structural Changes and Gross Pathology of Gut and associated Organs of Mice on Chronic Exposure to Pan Masala Plain and its Blend (Pan Masala Tobacco): A Review and an Experimental Study. Euroasian J Hepatogastroenterol 2012; 2 (2):94-97.

DOI: 10.5005/jp-journals-10018-1042

License: CC BY-NC 4.0

Published Online: 01-07-2012


Abstract

Swiss albino mice in three groups (control, experimental I and experimental II) of 60 each were fed staple diet (control), diet composed with 2% pan masala plain (PMP) and its blend (tobacco-PMT) for a period of up to 70 weeks with intermittent postmortem at 16, 56 and 70 weeks for assessing histological changes of the gut histopathology of mice. Mice of experimental groups I and II showed progressive advancement of structural changes of stomach during the study period. Fibrosis, thickening of prickle cells and also horney layered cells indicating lesions of acanthosis and hyperkeratosis initially and later prominently ulcers and papillomas which transformed into a peptic tumor occured. Spleen was found enlarged in mice fed with PMT. Animal feeding, hygiene, exploration and environmental care and health protocols were as per the standard guidelines and approved by National Institute of Occupational Health ethical committee. Here, chronic exposure of mice has been consistent in respect of feed and environment, unlike habitual PM users whose consumption is inconsistent depending on the body demand and choice on the variety of the blended PM product. This study endorses generating critical clinically designed case control, cohort data for awareness programs and PM substitutes as well as treatment to PM addicts.


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  1. Nigam SK, Bhatt HV. Chemical and experimental studies of plain and blended Indian pan masala. Proceeding of the National Academy of Sciences (India), 74th Annual Session, Jaipur, Section of Biological Sciences 2004 Dec 2-4:84. 2. Nigam SK, Bhatt HV. Analysis and experimental evaluation of Indian blended pan masala. Proceedings of 94th Indian Science Congress (A), Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu 2007 January;3-7
  2. Changing pattern of oral cavity lesions and personal habits over a decade: Hospital based record analysis from Allahabad. Indian J Comm Med 2009;34:321-25
  3. Pan masala: A new substance of dependence. Indian J Psychiat 1993:35:189
  4. Histopathology of chronic pan masala (PM) on the testis of mice. 76th Annual Session of National Academy of Sciences: Section of Biological Sciences (A:75, p:54), IIT&TIFR, Mumbai 2006 Oct:6-8
  5. Gutka or areca nut Chewer’s syndrome. Indian J Cancer 2009:46:170-72
  6. Pan masala advertisements are surrogate for tobacco products. Indian J Cancer 2005;42:94-98
  7. Long term exposure to Indian pan masala plain (PMP) and blend (PMT) causes gastro-hepatic injury in swiss albino mice. XII International Euroasian Congress of Surgery and Gastroenterology, (A), p138, Section: Diseases of Liver, Bile Ducts and Pancreas, Baki, 13-16, Oktyabr 2011, Baku: Azerbaijan Republic
  8. Svere iron deficiency anemia and gastrointestinal dysfunction associated with ingestion of pan masala. J Diet Suppl 2008;5:305-09
  9. Dominant lethal studies in mice exposed to blended indian pan masala. Annals of National Academy of Medical Sciences India 2004:40:91-96
  10. Effects of plain and blended indian pan masala on murine liver. Euroasian J Hepatogastoenterol 2011:1:27-29
  11. Effects of Indian pan masala (plain and blended - PMP & PMT) on testis after long-term feeding (PO) in mice. Int J Appl Biol pharmaec Tech 2012;3:235-41
  12. Oral sub-mucous fibrosis: Etiology, pathogenesis and future research bull. WHO 1994;72,856-996
  13. Long term carcinogenicity of pan masala in Swiss mice. Int J Cancer 1999;83:679-84
  14. Distribution of isomers of benzene hexachloride (BHC) and related histopathology of liver in hexachlorocyclohexane (HCCH) fed mice. Arch Env Hlth 1982;37:156-58
  15. Biochemical effects of chronic plain and blended indian pan masala (PM) on liver in mice 99th Proceedings of Indian Science Congress, KIIT University, Bhubhaeshwar: Abstract, Jan 3-7, 2012
  16. Effect of immunosuppressive agents on tumorogenesis in Swiss mice. Asian Med J 1982:25:809-15
  17. Relative effect of various chlorinated hydrocarbons on liver and kidney function in mice. Toxicol Appl Pharmacol 1966;9:139
  18. Unusually high levels of carcinogenic tobaccospecific nitrosamines in Sudan snuff (toombak). Carcinogenesis 199;12:1115-18
  19. The role of saliva borne mutagens and carcinogens in etiology of oral and esophageal carcinomas of betel nut and tobacco chewers. Carcinogens and Mutagens in the Environment (Vol. II). In: Stich HF, CRC Press, Boca Raton FL (Eds). Naturally Occurring Compounds 1983:44-58
  20. Ramachandani AG, DSouza AV, Berges AM, Bhisey RA. Evaluation of carcinogenic/cocarcinogenic activity of a common chewing product of pan masala in mouse skin, stomach and esophagus. Int J Cancer 1998:75:225-32
  21. Evaluation of pan masala for toxic effects on liver and other organs. Food Chem Toxicol 1992:30:161-63.
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