Background and aim: To find the degree of knowledge and awareness about hepatitis A virus (HAV) and hepatitis B virus (HBV). We also evaluated practices of vaccination among the medical students of Chittagong Medical College, Chittagong, Bangladesh. Materials and methods: A total of 125 students of Chittagong Medical College, 25 each from first year to 5th year were selected by stratified and simple random sampling. Information about knowledge, awareness and practices of hepatitis A and B vaccination was collected by selfadministered questionnaire along with direct observation of the respondents. Results: About 6.4% and 87% of the students were aware for vaccination against hepatitis A and hepatitis B respectively. None of the students had taken vaccine against hepatitis A whereas two-thirds of the students had been vaccinated for hepatitis B. Discussion and conclusion: Almost all students were unaware of vaccine against hepatitis A. In contrast, most of the students were aware about vaccine against hepatitis B. Despite the fact that vaccination program being priority National Public Health Program, vaccination procedures had not been implemented well in reality. Awareness of hepatitis A on preventive activities, such as increased utilization of safe water and handwashing practices should be strengthened.