Background/Objective: Hepatitis B virus (HBV) infection has remained a global public health challenge. Nigeria is in the high endemicity group with a prevalence of 8% or higher. The authors seized the opportunity of the 2010. World Hepatitis Day to mount a public enlightenment campaign and also screen civil servant in Enugu State of Nigeria for the infection. Materials and methods: In this cross-sectional study, civil servants attached to Government House, Enugu, Nigeria, were administered a structured questionnaire containing the putative risk factors for HBV transmission. They also underwent screening for hepatitis B surface antigen (HBsAg) in blood. Results: Out of 395 civil servants (206 males and 189 females) who participated in the screening, 36 were position for HBsAg giving a seroprevalence of 7.6%. Majority of the seropostive subjects (86.7%) were females (p < 0. 001). Among the risk factors examined, past history of native uvulectomy, sharing of toothbrush, sharing of razor blades and hair clippers were significantly more prevalent in the HBsAg positive group compared to the HBsAg negative subjects. In the females who had circumcision, the HBsAg seroprevalence was significantly higher than in the uncircumcized group (p = 0.014, odds ratio = 3.13). Conclusion: The seroprevalence of HBsAg in civil servants of Enugu State of Nigeria was 7.6%. More females than males were HBsAg seropositive and this may be related to previous circumcision in the females.